Modified bases in transfer RNA

Transfer RNA Methylases and Transfer RNA-methylated Bases of Polyoma Virus-transformed and Spontaneously Transformed Mouse Culture Cells1

culture system, which included nontumorigenic control cells and both spontaneously and polyoma virus-trans formed cells. Even when the growth rates of the three cell types were equalized, the transfer RNA methylase activ ities of the transformed cells were at least twofold greater than the activity of the control cells. A twoto fourfold relative increase in 3-methylcytosine was found in the tra...

Transfer RNA methylases and transfer RNA-methylated bases of polyoma virus-transformed and spontaneously transformed mouse culture cells.

culture system, which included nontumorigenic control cells and both spontaneously and polyoma virus-trans formed cells. Even when the growth rates of the three cell types were equalized, the transfer RNA methylase activ ities of the transformed cells were at least twofold greater than the activity of the control cells. A twoto fourfold relative increase in 3-methylcytosine was found in the tra...

Properties of a transfer RNA lacking modified nucleosides.

A transfer RNA complete devoid of modified nucleosides was synthesized by in vitro transcription, and some of its properties in aminoacylation and protein synthesis in vitro were studied. For this purpose, a plasmid was constructed which contained a glycine tRNA gene from Mycoplasma mycoides under the promoter of the T7 RNA polymerase, as well as a BstNI restriction site at the 3'-end of the tR...

A Modified Orcinol Reaction for RNA Determination

RNA-binding proteins: TRAPping RNA bases

In Bacillus subtilis, tryptophan biosynthesis is regulated by a mechanism called attenuation. The new crystal structure of the 'trp RNA binding attenuation protein', TRAP, in complex with RNA has provided new structural insights into how proteins can bind RNA to regulate transcription and translation.